Journal: Oxidative Medicine and Cellular Longevity
Article Title: Simvastatin Attenuates Abdominal Aortic Aneurysm Formation Favoured by Lack of Nrf2 Transcriptional Activity
doi: 10.1155/2020/6340190
Figure Lengend Snippet: Immune cell infiltration and increased VCAM1 level are associated with AAA formation in Nrf2 tKO mice. Mice of both genotypes represent the following groups: (1) sham (saline, n = 4), (2) angiotensin II (AngII group, n = 5), (3) simvastatin+saline (Sim group, n = 6), and (4) simvastatin+angiotensin II (Sim+AngII group, n = 6). Simvastatin was administered daily for 7 consecutive days before osmotic pump placement and during AngII infusion for another 14 days. (a) Quantification of circulating white blood cells and their subpopulations (LYM: lymphocytes; MON: monocytes; GRA: granulocytes). Three-way ANOVA with Tukey's post hoc test. (b) Relative expression of VCAM1 and SELE in the aortic wall. eEF2 was used as a reference gene. Three-way ANOVA with Tukey's post hoc test. (c) Relative expression of IL-1b , IL4 , and IL6 in the aortic wall. eEF2 was used as a reference gene. Three-way ANOVA with Tukey's post hoc test. Rectangle—mice which developed the aneurysm. (d) Immunofluorescent staining of infiltrating CD45 + and CD64 + cells within the aortic wall. CD45: yellow; CD64: red; nuclei: blue; L: lumen; magnification at 200x. Scale bar = 50 μ m. ∗ p < 0.05 and ∗∗∗ p < 0.001 vs. saline; # p < 0.05 and ### p < 0.001 vs. AngII; @ p < 0.05 and @@@ p < 0.001 vs. WT.
Article Snippet: After washing in PBS, samples were incubated overnight (4°C) with rabbit anti-AT1R polyclonal IgG antibodies (dilution 1 : 100; Abcam), rabbit anti-collagen I or collagen III IgG polyclonal antibodies (dilution 1 : 250; Abcam), rabbit anti-phospho-ERK1/2 IgG polyclonal antibody (dilution 1 : 100; Cell Signaling Technology), APC-conjugated rat antibody anti-mouse CD45 (BD, clone 30-F11), and PE-conjugated rat antibody anti-mouse CD64 (BD, Clone X54-5/7.1) diluted in 1% GS with 0.05% Tween-20.
Techniques: Expressing, Staining